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Polyubiqutin-B Antibody

Our Anti-Polyubiqutin-B mouse monoclonal primary antibody detects bovine, C. elegans, chicken, drosophila, and human Polyubiqutin-B, and is IgG. It is validated for use in IHC-Frozen, IHC-Paraffin-embedded, WB.

Product Specifications

Product Name Alternative

RPS27A; UBA52; UBB; UBC; Polyubiquitin-B; Polyubiquitin-C

UniProt

P0CG47

Reactivity

Bovine, Drosophila, Gallus, Human

Immunogen

Raised against purified ubiquitin conjugated with glutaraldehyde to keyhole limpet hemocyanin.

Target

Polyubiqutin-B

Clonality

Monoclonal

Clone

Ubi-1

Conjugation

Unconjugated

Dilution

WB: 1:500-1:1000; IHC: 1:500

Form

Lyophilized

Molecular Weight

8.5 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

This antibody can be used on mildly fixed histological sections of human brain for studies of Alzheimer's disease. This antibody also works on paraffin embedded material. It also recognizes other ubiquinated inclusion bodies such as Lewy bodies of Parkinson's disease and the Pick bodies in Pick's disease in formalin fixed tissues.

Tested Applications

IHC, WB

Host or Source

Mouse

Preservative

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Isotype

IgG1

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