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Neurofilament light polypeptide (NF-L) Antibody (DA2)

Our Anti-Neurofilament light polypeptide (NF-L) mouse monoclonal primary antibody detects bovine, horse, human, mouse, pig, and rat Neurofilament light polypeptide (NF-L), and is IgG. It is validated for use in FC, IF, ICC, IHC, WB.

Product Specifications

Product Name Alternative

NF-L; NF68; NEFL; Neurofilament light polypeptide; Neurofilament protein; Neurofilament triplet L protein; NFL

UniProt

P07196

Reactivity

Bovine, Equine, Human, Mouse, Porcine, Rat

Immunogen

The antibody has been made against enzymatically dephosphorylated, full length, pig NF-L protein. The antibody binding epitope has been mapped to a short peptide in the C-terminal “tail” region of the molecule within the sequence YYTSHVQEEQIEVEETIEA, amino acids 441-460 of the human sequence.

Target

Neurofilament light polypeptide (NF-L)

Clonality

Monoclonal

Clone

DA2

Conjugation

Unconjugated

Dilution

WB: 1:5000; IHC: 1:2000; ICC: 1:1000

Form

Lyophilized

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

A dilution of 2 ug per 10^6 cells is recommended for FC.

Tested Applications

FC, ICC, IF, IHC, WB

Host or Source

Mouse

Preservative

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Isotype

IgG1

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