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Myelin Basic Protein (MBP) Antibody

Our Anti-Myelin basic protein (MBP) mouse monoclonal primary antibody detects bovine, human, and rat Myelin basic protein (MBP), and is IgG. It is validated for use in ICC, IHC-Frozen, WB.

Product Specifications

Product Name Alternative

Myelin Basic Protein; Myelin A1 protein; Myelin membrane encephalitogenic protein; MBP

UniProt

P02686

Reactivity

Bovine, Human, Rat

Immunogen

Three peptide sequences conserved in higher verterbrate MBP protein.

Target

Myelin basic protein (MBP)

Clonality

Monoclonal

Clone

7G7

Conjugation

Unconjugated

Dilution

WB: 1:5000-1:10000; IHC: 1:500-1:1000; ICC: 1:500-1:1000

Form

Lyophilized

Molecular Weight

21.5 kDa and 18.5 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

IH (P) 1:500-1:1000 and Flow Cytometry (~2 ug per10^6 cells) . Material should not be over fixed; 2-3 hour post-fixing time is recommended. Long fixations can effect reactivity. In paraffin citrate acid treatment for antigen recovery is recommended.

Tested Applications

ICC, IHC, WB

Host or Source

Mouse

Preservative

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide

Isotype

IgG

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