Integrin beta 2 alpha subunit (CD11b/c, Mac-1) Antibody

Immunoprecipitates three polypeptides of 160 kDa, 103 kDa, and 95 kDa and a fainter band may also be seen at 133 kDa under non-reducing conditions. If the immunoprecipitated proteins are reduced, two major peptides of 163 kDa and 100 kDa and a minor 135 kDa peptide are seen.

FC: Flow Cytometry: Unfixed cells preferred, acetone fixed or quickly fixed 1% PLP fixed cells can be used. IHC: Immunohistochemical studies of rat fresh frozen tissue sections and paraffin-embedded tissue sections following either periodate-lysine-paraformaldehyde (PLP) fixation, or acetone. Works on very lightly PFA fixed, frozen tissues. (perfusion only 4% PFA 10-15' no post-fix) . Epitope can be sensitive to fixation. Dilutions detection method dependent 1:100 to 1:200 recommended. ICC: Unfixed preferred, or acetone fixed cells; 5-10', 2% PLP fixed cells, 1-2 μg/mL. Dilution is detection method dependent. Immunoprecipitation: use rabbit anti-mouse or anti-mouse IgG beads for capture only. The use of Protein A or Protein G is not recommended. 1-5 μg/mL in restricted volumes. Clone does not work in traditional reduced Westerns. Use immunoprecipitation to resolve reactive protein bands.