NGFR/p75 Neurotrophin Receptor (p75NTR) Antibody

IHC (lightly fixed), ELISA, WB, Flow Cytometry (2 ug per 10^6 cells) IP (non-reducing conditions only!; do not use reducing agents such as DTT or beta-mercaptoethanol), Traditional formalin fixed paraffin embedded immunohistochemistry is NOT recommended with MC192. Motor neuron isolation, Gene/Toxin Delivery to rat sensory/motor neurons. A working solution of 1-2 μg/mL was determined by immunohistochemical staining on 4% paraformaldehyde fixed, or alcohol fixed rat spinal cord and brain. For non-denatured WB, 1-5 μg/mL was found to be suitable with suitable controls (PC12 lysate) . ELISA: detection only, 1-5 μg/mL has been suggested in literature. Immunoprecipitation: 5 μg/mL, > 0.5% triton X-100 buffer/500 ug/lysate; PC12 positive control strong suggested. MC192 is not suitable as a blocking agent, although it has been incorrectly used for this purpose in many published works. The antibody was generated specifically by screening for monoclonals that had the ability to ENHANCE the binding of NGF, the natural ligand for p75. Therefore, this antibody is particularly unusual. The full details can be found in the original paper, which is listed on our datasheet (see Chandler et al, 1984) .