Prion Protein Antibody

Antibodies were prepared by injecting laying hens with purified recombinant human PRN protein. After a series of boosts, eggs were collected from hyperimmunized aniamsl and the IgY fraction prepared. Affinity purified antibodies were then isolated from the IgY fraction using a PRN protein column. Finally, affinity purified antibodies were mixed with the IgY fraction to make the final product. These antibodies have been validated with human, mouse and rat tissues.

Product Specifications

Product Name Alternative

Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)

UniProt

P04156

Reactivity

Human, Mouse, Rat

Immunogen

Reccombinant fragment of human prion

Target

Prion Protein (PRN)

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10 mg/mL

Dilution

WB: 1:2500-1:5000; IHC: 1:1000-1:2000; ICC: 1:1000-1:2000

Form

Liquid

Molecular Weight

27 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

ICC, IHC, WB

Host or Source

Gallus

Preservative

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.