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Glial fibrillary acidic protein (GFAP) Antibody

Our Anti-Glial fibrillary acidic protein (GFAP) chicken polyclonal primary antibody detects cat, human, mouse, other mammals (predicted), and rat Glial fibrillary acidic protein (GFAP), and is IgY preparation. It is validated for use in ICC, IHC-Frozen, IHC-Paraffin-embedded, WB.

Product Specifications

Product Name Alternative

Astrocyte; Glial fibrillary acidic protein; GFAP

UniProt

Q28115

Reactivity

Feline, Human, Mouse, Rat

Immunogen

Recombinant GFAP (expressed in E.coli) and native bovine GFAP

Target

Glial fibrillary acidic protein (GFAP)

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 1:5000; ICC: 1:1000-1:5000

Form

Lyophilized

Molecular Weight

50 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ICC: Using fluorescent secondary antibodies or peroxidase or other enzyme-linked methods is recommended on 4% PFA fixed cells in culture, with 3 hr-o/n incubation of primary antibody. IHC: 4% PFA frozen tissues, permeabilized. IHC (paraffin-embedded) : capable, HEIR treatment typically necessary. Biosensis recommends optimal dilutions/concentrations should be determined by the end user.

Prediction Reactivity

Mammal

Tested Applications

ICC, IHC, WB

Host or Source

Gallus

Preservative

Lyophilized IgY preparation, with sodium azide.

Isotype

IgY

Frequently Asked Questions

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