Actin (Nitrocellulose Membrane Loading Control) Antibody

Our anti-actin antibody (made against rabbit muscle actin) recognizes mouse, rat and human actin proteins in SDS-polyacrylamide gel transfers onto nitrocellulose membranes. This loading control assures that similar amounts of total proteins were added to each of the lanes in the gels. Note that this product does not work in transfers using PVDF membranes.

Product Specifications

UniProt

P63267

Reactivity

Human, Mouse, Rat

Immunogen

Actin purified from rabbit muscle

Target

Actin

Clonality

Polyclonal

Conjugation

Unconjugated

Concentration

10 mg/mL

Dilution

WB Brain: 1:1000-1:2000

Form

Liquid

Molecular Weight

42 kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Tested Applications

Host or Source

Gallus

Preservative

Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.