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CDYL Antibody

Goat polyclonal antibody to CDYL

Product Specifications

Product Name Alternative

Anti CDYL antibody, anti chromodomain protein, Y-like antibody, anti CDYL1, DKFZP586C1622, MGC131936 antibody, anti CDY-like, autosomal antibody, anti bA620A17.2 (chromodomain protein, Y chromosome-like) antibody, anti chromodomain protein, Y chromosome-like antibody, anti chromodomain protein, Y chromosome-like, isoform b antibody, anti testis-specific chromodomain Y-like protein antibody, anti DKFZp586C1622 antibody

Reactivity

Human

Target

CDYL

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

SMLKYLQRKIDEF

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, WB: 1-3 μg/ml

Molecular Weight

60.6; 45.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99 (13) :8707-12; PMID: 12072557) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Porcine, Rat

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

9425

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