CDYL Antibody
Goat polyclonal antibody to CDYL
Product Specifications
Product Name Alternative
Anti CDYL antibody, anti chromodomain protein, Y-like antibody, anti CDYL1, DKFZP586C1622, MGC131936 antibody, anti CDY-like, autosomal antibody, anti bA620A17.2 (chromodomain protein, Y chromosome-like) antibody, anti chromodomain protein, Y chromosome-like antibody, anti chromodomain protein, Y chromosome-like, isoform b antibody, anti testis-specific chromodomain Y-like protein antibody, anti DKFZp586C1622 antibody
Reactivity
Human
Target
CDYL
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
SMLKYLQRKIDEF
Field of Research
Stem Cell & Developmental Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:4000, WB: 1-3 μg/ml
Molecular Weight
60.6; 45.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 75kDa band observed in Human Testes lysates (calculated MW of 66.5kDa according to NP_004815.2) . The observed molecular weight corresponds to earlier findings in literature with different antibodies (Lahn et al , Proc Natl Acad Sci U S A. 2002 Jun 25;99 (13) :8707-12; PMID: 12072557) . Recommended concentration: 1-3 μg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Porcine, Rat
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9425
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