Goat anti-UBE2C / UBCH10 Antibody

Anti UBE2C antibody, anti UBCH10 antibody, anti dJ447F3.2 antibody, anti ubiquitin-conjugating enzyme E2C antibody, anti ubiquitin-protein ligase C antibody, anti ubiquitin carrier protein E2-C antibody, anti cyclin-selective ubiquitin carrier protein antibody, anti mitotic-specific ubiquitin-conjugating enzyme antibody, anti RP3-447F3.1 antibody

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approximately 20KDa band was detected in mitotic (i.e Nocodazole arrested) HeLa whole cell lysates Recommended for use at 1-3 μg/ml. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates using protein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, with very faint or no signal in G1 and S phase cels (fixed using paraformaldehyde, primary concentration 2.5 μg/ml, using FITC anti-Goat)

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.