PI31/PSMF1 (Isoform 1) Antibody
Goat polyclonal to PI31
Product Specifications
Product Name Alternative
Anti PSMF1 antibody, anti PI31 antibody, anti proteasome (prosome, macropain) inhibitor subunit 1 (PI31) antibody, anti RP4-545L17.1 antibody, anti proteasome inhibitor hP131 subunit antibody, anti proteasome inhibitor subunit 1 antibody
Reactivity
Human, Mouse, Rat
Target
PI31 / PSMF1 (Isoform 1)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
DHLPPPGYDDMYL
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 0.3-1μg/ml, IF/ICC: 10μg/ml
Molecular Weight
29.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2) .ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 29.8kDa according to NP_006805.2) . In transfected HEK293 transiently expressing PSMF1 a band of approx. 35kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/ml.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, IF, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9491
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