IRF5 Antibody
Goat polyclonal antibody to IRF5
Product Specifications
Product Name Alternative
Anti IRF5 antibody, anti interferon regulatory factor 5 antibody, anti SLEB10 antibody
Reactivity
Human
Target
IRF5
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QGPWPMHPAGMQ
Field of Research
Infectious Disease & Virology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx. 55+60kDa bands observed in Human Spleen lysates, approx. 65kDa in lysates of cell line A549, and approx. 70kDa in lysates of cell lines Daudi (calculated MW of 57.8kDa according to NP_001092099.1) . These molecular weights have been observed by other commercial sources, and were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-2μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5μg/ml.
Molecular Weight
56.0; 56.0; 57.8
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Prediction Reactivity
Bovine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
3663
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