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HA Tag Antibody

Rabbit polyclonal antibody to DYKDDDDK Tag is used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest is an amino-terminal or carboxy-terminal fusion. It is a useful tool for the localization and characterization of DDDDK tagged proteins.

Product Specifications

Product Name Alternative

Anti-Tag from influenza hemagglutinin protein antibody

Immunogen

Synthetic Peptide

Target

KLH conjugated synthetic peptide encoding HA tag (CYPYDVPDYAYPYDVPDYA) was used as antigen.

Clonality

Polyclonal

Conjugation

Unconjugated

Dilution

WB: 1:1000, IF/ICC: 1:10-50, ChIP: 1:100

Form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

References & Citations

Simkin, Dina et al. Andersen's syndrome mutants produce a knockdown of inwardly rectifying K+ channel in mouse skeletal muscle in vivo Cell Tissue Res, 371, 309-323 (2018)

Pubmed id

29018970

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles

Notes

For research use only.

Tested Applications

ChIP, IF, WB

Host or Source

Rabbit

Isotype

Rabbit IgG

Available Sizes

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