Recombinant Human MMP-9
Product Specifications
Product Name Alternative
92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9
UniProt
P14780
Expression Region
20-707
Host
HEK293 cells
Tag
C-terminal His Tag
Applications
Western Blot, ELISA
Field of Research
Cancer, Cardiovascular, Metabolism
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >700 pmol/min/µg, The proenzyme needs to be activated by APMA for an activated form.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
93 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Organism Species
Human
Available Sizes
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