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Recombinant Human MMP-9

Product Specifications

Product Name Alternative

92 kDa gelatinase, 92 kDa type IV collagenase, CLG4B, Gelatinase B, GELB, Matrix metalloproteinase 9, MMP 9, MMP9

UniProt

P14780

Expression Region

20-707

Host

HEK293 cells

Tag

C-terminal His Tag

Applications

Western Blot, ELISA

Field of Research

Cancer, Cardiovascular, Metabolism

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >700 pmol/min/µg, The proenzyme needs to be activated by APMA for an activated form.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

93 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.

Formulation

Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.

Organism Species

Human

Available Sizes

Frequently Asked Questions

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