Anti-Bcl-2 (Phospho-Ser70) Antibody

Rabbit Polyclonal Antibody specific to Bcl-2 (Phospho-Ser70)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Bcl-2 (Phospho-Ser70) Rabbit Polyclonal Antibody

Gene Name

BCL2

Gene ID

151430

NCBI Gene ID

UniProt

P10415

Cellular Locus

Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of Serine 70 (R-T-S(p)-P-L) derived from human Bcl-2 and conjugated to KLH.

Target Antigen

Apoptosis regulator Bcl-2

Target

Bcl-2 (Phospho-Ser70)

Clonality

Polyclonal

Type

Antibody

Applications

IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). {PubMed:1508712, PubMed:17418785, PubMed:18570871, PubMed:20889974, PubMed:21358617, PubMed:8183370, PubMed:11368354}.

Additionnal Information

Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their application.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human Bcl-2 only when phosphorylated at serine 70.Accession no.: P10415, NP_000624.2

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspase. Bcl-2 inhibits caspase acativity either by preventing release of cytochrome c from the mitochondria and/or by binding to apoptosis-activating factor (Apaf-1). The predicted molecular weight of Bcl-2 is ~26kDa.