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Recombinant Murine Mucosae-associated Epithelial Chemokine/CCL28

Selected Variant

AP60362

Size: Inquire

Product Specifications

Background

CCL28 belongs to CC family chemokine, also named mucosae-associated epithelial chemokine (MEC) . The CCL28 mRNA is highly expressed by epithelial cells of diverse tissues including normal and pathological colon, salivary gland, mammary gland, trachea and rectum. The amino acids sequence of human and mouse CCL28 shares 83% identity, when the protein is processed to be the mature form. CCL28 is signaling through CCR3 and CCR10, and chemotactic for resting CD4, CD8 T-cells and eosinophils.

Product Name Alternative

CCK1

Gene ID

56838

UniProt

Q9JIL2

Target

SEAILPMASS CCTEVSHHVS GRLLERVSSC SIQRADGDCD LAAVILHVKR RRICISPHNR TLKQWMRASE VKKNGRENVC SGKKQPSRKD RKGHTTRKHR TRGTHRHEAS R

Purification

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin

Less than 1 EU/ugof rMuMEC/CCL28 as determined by LAL method.

Bioactivity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine lymphocytes is in a concentration range of 1.0-10.0 ng/ml.

Storage Conditions

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculated Molecular Weight

Approximately 12.6 kDa, a single, non-glycosylated polypeptide chain containing 111 amino acids.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Host or Source

Escherichia coli

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