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Goat anti-Dysadherin Antibody

Goat polyclonal antibody to FXYD5

Product Specifications

Product Name Alternative

Anti FXYD5 antibody, anti dysadherin antibody, anti FXYD domain containing ion transport regulator 5 antibody, anti RIC antibody, anti IWU1 antibody, anti IWU-1 antibody, anti dysad antibody, anti HSPC113 antibody, anti KCT1 antibody, anti OIT2 antibody, anti PRO6241 antibody, anti FXYD domain-containing ion transport regulator 5 antibody, anti keratinocytes associated transmembrane protein 1 antibody, anti DYSAD antibody, anti HSPC113 antibody

Reactivity

Human

Target

Dysadherin

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GKCRQLSRLCRNHCR

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000

Molecular Weight

19.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Preliminary experiments gave an approx 38 kDa band in Human Spleen lysate and an approx 26 kDa in Human Lung lysate after 1 μg/ml antibody staining. The bands were successfully blocked by incubation with the immunizing peptide. Please note that according to the literature this protein is O-glycosylated (PMID: 12672699), which may explain the different sizes of bands we observe given the calculated size of 19.5 kDa according to NP_054883 and NP_659003. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

53827

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