ELMO1 Antibody
Goat polyclonal antibody to ELMO1
Product Specifications
Product Name Alternative
Anti ELMO1 antibody, anti engulfment and cell motility 1 (ced-12 homolog, C. elegans) antibody, anti CED12 antibody, anti KIAA0281 antibody, anti ced-12 homolog 1 antibody, anti engulfment and cell motility 1 antibody, anti CED-12 antibody, anti ELMO-1 antibody, anti MGC126406 antibody, anti OTTHUMP00000207056 antibody, anti OTTHUMP00000207060 antibody
Reactivity
Human, Mouse, Rat
Target
ELMO1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
PKEPSNYDFVYDCN
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1-0.3 μg/ml
Molecular Weight
83.8; 28.7
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2 μg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa) . Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].
Prediction Reactivity
Bovine, Canine, Zebrafish
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
9844
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