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ELMO1 Antibody

Goat polyclonal antibody to ELMO1

Product Specifications

Product Name Alternative

Anti ELMO1 antibody, anti engulfment and cell motility 1 (ced-12 homolog, C. elegans) antibody, anti CED12 antibody, anti KIAA0281 antibody, anti ced-12 homolog 1 antibody, anti engulfment and cell motility 1 antibody, anti CED-12 antibody, anti ELMO-1 antibody, anti MGC126406 antibody, anti OTTHUMP00000207056 antibody, anti OTTHUMP00000207060 antibody

Reactivity

Human, Mouse, Rat

Target

ELMO1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

PKEPSNYDFVYDCN

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml

Molecular Weight

83.8; 28.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa and 50kDa bands seen in Jurkat cell lysate. Recommended for use at 1-2 μg/ml. Note the smaller isoform of this protein has a consensus glycosylation site, which may explain the higher than expected band size (50 kDa versus 30 kDa) . Glycosylation can affect migration of proteins on SDS-PAGE [Hames, B.D and Rickwood, D., eds. Gel electrophoresis of proteins: a practical approach. Oxford University Press].

Prediction Reactivity

Bovine, Canine, Zebrafish

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

9844

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