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Goat anti-COP1 / PSEUDO-ICE (Isofom 2) Antibody

Goat polyclonal antibody to CARD16

Product Specifications

Product Name Alternative

Anti CARD16 antibody, anti COP antibody, anti CARD only protein antibody, anti PSEUDO-ICE antibody, anti COP1 antibody, anti pseudo interleukin-1beta converting enzyme antibody

Reactivity

Human

Target

COP1 / PSEUDO-ICE (Isofom 2)

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

ETLGLSAGPIPGN

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:4000, IHC-P: 10ug/ml

Molecular Weight

10.7

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Preliminary experiments gave an approx 28-30kDa band in human colon, pancreas, placenta and tonsil lysates after 0.3 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 10.7kDa according to NP_443121.1. The 28-30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Tested Applications

ELISA, FC, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

114769

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