NFM (Neurofilament Medium Chain) Antibody
NFM protein was purified from bovine spinal cords following the method of Delacourte et al. ["Study of the 10-nm-filament fraction isolated during the standard microtubule prepartion" (1980) . Biochem. J. 191 (2) : 543-546], followed by Prepcell purification (Bio-Rad) . Antibodies were prepared by injecting purified NFM protein into laying hens and purifying the IgY fraction from eggs collected from hyper-immunized hens. After several boosts, hens were co-boosted with recombinant NFM protein expressed in bacteria. These antibodies have been validated with human, mouse and rat tissues.
Product Specifications
Product Name Alternative
Neurofilament medium polypeptide (NF-M) (160 kDa neurofilament protein) (Neurofilament 3) (Neurofilament triplet M protein)
UniProt
P07197
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant construct containing the C-terminus of the human sequence expressed in Escherichia coli
Target
Neurofilament Medium Chain (NFM)
Clonality
Polyclonal
Conjugation
Unconjugated
Concentration
2 mg/mL
Dilution
WB: 1:1000-1:2000; IHC: 1:1000-1:2000; ICC: 1:1000-1:2000
Form
Liquid
Molecular Weight
150 kDa
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Tested Applications
ICC, IHC, WB
Host or Source
Gallus
Preservative
Isotype
IgY
Frequently Asked Questions
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