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PRAM1 Antibody

Goat polyclonal to PRAM1

Product Specifications

Product Name Alternative

Anti PRAM-1 antibody, anti PRAM-1 protein antibody, anti PML-RARA target gene encoding an Adaptor Molecule-1 antibody, anti PML-RARA regulated adaptor molecule 1 antibody, anti MGC39864 antibody, anti PML-RAR antibody

Reactivity

Human

Target

PRAM1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

DFCDPLENQPLPLGR

Field of Research

Epigenetics

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:8000, WB: 1-3 μg/ml

Molecular Weight

74

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3 μg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.

Prediction Reactivity

Porcine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

84106

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