Calcium Orange AM

Calcium Orange AM is an intracellular calcium reporter. Specific fluorescence can be detected when free calcium binds to Calcium Orange AM (Ex/Em=549/576 nm) . Calcium Orange AM does not enter the vacuoles and does not compartmentalize into acidic vesicles[1].

Product Specifications

CAS Number

[172646-19-4]

UNSPSC

12171500

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/calcium-orange-am.html

Purity

98.00

Solubility

10 mM in DMSO

Smiles

CN(C1=CC2=[O+]C3=C(C=CC(N(C)C)=C3)C(C4=CC=C(NC(NC5=CC=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C(OCCOC6=CC=CC=C6N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C5)=S)C=C4C([O-])=O)=C2C=C1)C

Molecular Formula

C59H62N6O21S

Molecular Weight

1223.22

References & Citations

[1]Lam CM, et al. Monitoring cytosolic calcium in the dinoflagellate Crypthecodinium cohnii with calcium orange-AM. Plant Cell Physiol. 2005 Jun;46 (6) :1021-7.|[2]Inami W, et al. Intracellular calcium ion concentration measurement using a phase-modulation fluorescence lifetime method with compensation for phase shift due to the presence of proteins. Anal Sci. 2013;29 (2) :199-203.