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Goat anti-EGFR Antibody

Goat polyclonal antibody to EGFR

Product Specifications

Product Name Alternative

Anti EGFR antibody, anti epidermal growth factor receptor antibody, anti erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian antibody, anti HGNC:3236 antibody, anti ERBB antibody, anti ERBB1 antibody, anti mENA antibody, anti avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, anti epidermal growth factor receptor antibody, anti epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) antibody, anti truncated epidermal growth factor receptor antibody

Reactivity

Human

Target

EGFR

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

QKGSHQISLDNPD

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.1-0.3 μg/ml

Molecular Weight

134

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2) . The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

1956

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