Goat anti-EGFR Antibody
Goat polyclonal antibody to EGFR
Product Specifications
Product Name Alternative
Anti EGFR antibody, anti epidermal growth factor receptor antibody, anti erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian antibody, anti HGNC:3236 antibody, anti ERBB antibody, anti ERBB1 antibody, anti mENA antibody, anti avian erythroblastic leukemia viral (v-erb-b) oncogene homolog antibody, anti epidermal growth factor receptor antibody, anti epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene homolog) antibody, anti truncated epidermal growth factor receptor antibody
Reactivity
Human
Target
EGFR
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
QKGSHQISLDNPD
Field of Research
Cell Biology
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.1-0.3 μg/ml
Molecular Weight
134
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 170kDa band observed in lysates of cell line HeLa (calculated MW of 134kDa according to NP_005219.2) . The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.1-0.3μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
1956
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