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ATP5A1 Antibody

Goat polyclonal antibody to ATP5A1

Product Specifications

Product Name Alternative

Anti ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, anti ATP5A antibody, anti ATP5AL2 antibody, anti ATPM antibody, anti MOM2 antibody, anti hATP1 antibody, anti ATP synthase alpha chain, mitochondrial antibody, anti ATP synthase subunit alpha, mitochondrial antibody, anti ATP synthase, H+ transportin antibody

Reactivity

Human, Mouse, Porcine, Rat

Target

ATP5A1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

RVHGLRNVQAEE

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.01-0.03 μg/ml

Molecular Weight

59.8; 57.5; 54.5

References & Citations

Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)

Pubmed id

30377371

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1) . Recommended concentration: 0.01-0.03μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Prediction Reactivity

Bovine, Canine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

498

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