ATP5A1 Antibody
Goat polyclonal antibody to ATP5A1
Product Specifications
Product Name Alternative
Anti ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle antibody, anti ATP5A antibody, anti ATP5AL2 antibody, anti ATPM antibody, anti MOM2 antibody, anti hATP1 antibody, anti ATP synthase alpha chain, mitochondrial antibody, anti ATP synthase subunit alpha, mitochondrial antibody, anti ATP synthase, H+ transportin antibody
Reactivity
Human, Mouse, Porcine, Rat
Target
ATP5A1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
RVHGLRNVQAEE
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.01-0.03 μg/ml
Molecular Weight
59.8; 57.5; 54.5
References & Citations
Sikorski, Krzysztof et al. A high-throughput pipeline for validation of antibodies Nat Methods, 15, 909-912 (2018)
Pubmed id
30377371
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1) . Recommended concentration: 0.01-0.03μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
498
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