Calreticulin Antibody
Goat polyclonal antibody to CALR.
Product Specifications
Product Name Alternative
Anti calreticulin antibody, anti CRT antibody, anti RO antibody, anti SSA antibody, anti cC1qR antibody, anti CRP55 antibody, anti ERp60 antibody, anti HACBP antibody, anti Sicca syndrome antigen A (autoantigen Ro antibody, anti calreticulin) antibody, anti calregulin antibody, anti endoplasmic reticulum resident protein 60 antibody, anti grp60 antibody
Reactivity
Human, Rat
Target
Calreticulin
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
HPEIDNPEYSPDPS
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:128000, WB: 0.01-0.03 μg/ml
Molecular Weight
48.1
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 60kDa band observed in Human and Rat Lung lysates (calculated MW of 48.1kDa according to NP_004334.1) . These bands correspond to earlier findings in literature with different antibodies (Conway et al, J Biol Chem. 1995 Jul 14;270 (28) :17011-6. PMID:. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Prediction Reactivity
Bovine, Canine, Mouse, Porcine
Tested Applications
ELISA, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
811
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