STAT5A (aa681-692) Antibody
Goat polyclonal antibody to STAT5A
Product Specifications
Product Name Alternative
Anti MGF antibody, anti signal transducer and activator of transcription 5A antibody, anti STAT5 antibody, anti STAT5A antibody
Reactivity
Human
Target
STAT5A (aa681-692)
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
KYYTPVLAKAVD
Field of Research
Epigenetics
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:8000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
Molecular Weight
90.6
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
WB: Approx 100kDa band observed in lysates of cell line K562 (calculated MW of 90.6kDa according to NP_003143.2) . Recommended concentration: 0.1-0.3μg/ml. Primary incubation 1 hour at room temperature. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5μg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, FC, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
6776
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