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NR5A2/LRH1 Antibody

Goat polyclonal antibody to NR5A2

Product Specifications

Product Name Alternative

Anti NR5A2 antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody, anti B1-binding factor antibody, anti B1F antibody, anti B1F2 antibody, anti CPF antibody, anti CYP7A promoter-binding factor antibody, anti FTF antibody, anti FTZ F1 antibody, anti FTZ F1beta antibody, anti FTZ-F1 antibody, anti FTZ-F1beta antibody, anti hB1F 2 antibody, anti HB1F antibody, anti hB1F-2 antibody, anti Hepatocytic transcription factor antibody, anti Liver receptor homolog 1 antibody, anti LRH-1 antibody, anti LRH1 antibody, anti Nr5a2 antibody, anti NR5A2_HUMAN antibody, anti Nuclear receptor subfamily 5 group A member 2 antibody

Reactivity

Human, Porcine

Target

NR5A2 / LRH1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

TDYDRSPFVTSPIS

Field of Research

Stem Cell & Developmental Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 55kDa band observed in nuclear lysates of cell lines HeLa and HepG2, and approx 60kDa band observed in Pig Ovary lysates (calculated MW of 56.5kDa according to Human NP_003813.1 and Pig AFD98842.1) . Recommended concentration: 0.3-1μg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Liver and NIH-3T3 cell line for this particular batch. Immunohistochemistry: This antibody has been successfully used in IHC on Mouse.

Molecular Weight

61.3; 56.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

2494

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