Thrombospondin Antibody

Goat polyclonal antibody to THBS1.

Product Specifications

Product Name Alternative

Anti THBS1 antibody, anti thrombospondin 1 antibody, anti THBS antibody, anti TSP antibody, anti TSP1 antibody, anti thrombospondin-1p180 antibody

Reactivity

Human, Mouse

Target

Thrombospondin

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NRIPESGGDNSVFD

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml

Molecular Weight

129

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

WB: Approx 150kDa band observed in Mouse Kidney lysates (calculated MW of 129kDa according to NP_035710.2) . Recommended concentration: 0.3-1μg/ml. Primary incubation 1 hour at room temperature.. IHC: Paraffin embedded Human Spleen. Recommended concentration: 5μg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and HepG2 cells. Recommended concentration: 10μg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Prediction Reactivity

Bovine, Rat

Tested Applications

ELISA, FC, IF, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.