5-Methyluridine

5-Methyluridine (m5U) is an RNA modified nucleotide generated by RNA methyltransferases (such as TrmA and RumA), which mainly targets specific uracil sites in RNA molecules such as the T arm of tRNA and rRNA. 5-Methyluridine relies on enzyme recognition of RNA secondary/tertiary structures (such as the T loop of tRNA or the specific stem-loop structure of rRNA) and participates in physiological processes such as translation accuracy and ribosome function by stabilizing RNA folding or regulating base pairing[1][2][3].

Product Specifications

CAS Number

[1463-10-1]

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Endogenous Metabolite

Type

Natural Products

Related Pathways

Metabolic Enzyme/Protease

Applications

Metabolism-protein/nucleotide metabolism

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/5-Methyluridine.html

Concentration

10mM

Purity

99.88

Solubility

DMSO : ≥ 100 mg/mL|H2O : 100 mg/mL (ultrasonic)

Smiles

O[C@H]1[C@@H](O)[C@H](N2C(NC(C(C)=C2)=O)=O)O[C@@H]1CO

Molecular Formula

C10H14N2O6

Molecular Weight

258.23

Precautions

H302, H315, H319, H335

References & Citations

[1]Hur S, et al. Substrate recognition by RNA 5-methyluridine methyltransferases and pseudouridine synthases: a structural perspective. J Biol Chem. 2006 Dec 22;281 (51) :38969-73.|[2]Jones JD, et al. Conserved 5-methyluridine tRNA modification modulates ribosome translocation. bioRxiv [Preprint]. 2023 Nov 12:2023.11.12.566704.|[3]Yang TH, et al. Intracellular levels of S-adenosylhomocysteine but not homocysteine are highly correlated to the expression of nm23-H1 and the level of 5-methyldeoxycytidine in human hepatoma cells with different invasion activities. Nutr Cancer. 2006;55 (2) :224-31.