5(6)-FITC
Product Specifications
UNSPSC Description
5(6)-FITC (Fluorescein 5(6)-isothiocyanate) is an amine-reactive derivative of a fluorescent dye, characterized by high absorbance and excellent fluorescence quantum yield. The isothiocyanate group of FITC can react with various functional groups on proteins, including amines, thiols, imidazoles, tyrosines and carbonyls, enabling the labeling of proteins such as antibodies and lectins. 5(6)-FITC has a wide range of applications, including flow cytometry, immunofluorescence, protease assays and conjugation. The maximum excitation/emission wavelengths are 492/518 nm[1][2][3][4].
Target Antigen
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/5_6_-FITC.html
Solubility
DMSO : ≥ 156 mg/mL
Smiles
OC1=CC=C(C2(O3)C(C=C(N=C=S)C=C4)=C4C3=O)C(OC5=C2C=CC(O)=C5)=C1.OC6=CC=C(C7(O8)C(C=CC(N=C=S)=C9)=C9C8=O)C(OC%10=C7C=CC(O)=C%10)=C6
Molecular Weight
389.38
References & Citations
[1]J S Miller , ET AL. Flow cytometric identification of microorganisms by dual staining with FITC and PI. Cytometry. 1990;11(6):667-75.|[2]S S Twining, et al. Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes.Anal Biochem. 1984 Nov 15;143(1):30-4.|[3]K Sugiura, et al. A simple quantitative immunofluorescence assay for FITC-labeled antibody on platelets. J Immunol Methods. 1981;40(2):165-70.|[4]Natalia Oddone, et al. Cell uptake mechanisms of PAMAM G4-FITC dendrimer in human myometrial cells. J Nanopart Res. 2013 15:1776.
Shipping Conditions
Blue Ice
Product Datasheet
http://file.medchemexpress.com/batch_PDF/HY-15941/5-6-FITC-DataSheet-MedChemExpress.pdf
Product MSDS
http://file.medchemexpress.com/batch_PDF/HY-15941/5-6-FITC-SDS-MedChemExpress.pdf
Clinical Information
No Development Reported
CAS Number
27072-45-3
Available Sizes
Curated Selection
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