DFHBI
DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP) . DFHBI can be used for imaging RNA in living cells. . Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells (Ex/Em = 469/501 nm) .
Product Specifications
CAS Number
1241390-29-3
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/DFHBI.html
Purity
99.13
Solubility
DMSO : ≥ 83.33 mg/mL
Smiles
O=C(N(C)C(C)=N/1)C1=C\C2=CC(F)=C(O)C(F)=C2
Molecular Formula
C12H10F2N2O2
Molecular Weight
252.22
Precautions
H315, H319, H335
References & Citations
[1]Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136 (4) :1198-201.|[2]Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9 (1) :146-55.|[3]Paige JS, et al. RNA mimics of green fluorescent protein. Science. 2011 Jul 29;333 (6042) :642-6.
Shipping Conditions
Room Temperature
Storage Conditions
4°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Available Sizes
Frequently Asked Questions
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