DFHBI

DFHBI is a small molecule that resembles the chromophore of green fluorescent protein (GFP) . DFHBI can be used for imaging RNA in living cells. . Spinach and DFHBI are essentially nonfluorescent when unbound, whereas the Spinach-DFHBI complex is brightly fluorescent both in vitro and in living cells (Ex/Em = 469/501 nm) .

Product Specifications

CAS Number

[1241390-29-3]

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/DFHBI.html

Purity

99.13

Solubility

DMSO : ≥ 83.33 mg/mL

Smiles

O=C(N(C)C(C)=N/1)C1=C\C2=CC(F)=C(O)C(F)=C2

Molecular Formula

C12H10F2N2O2

Molecular Weight

252.22

Precautions

H315, H319, H335

References & Citations

[1]Song W, et al. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136 (4) :1198-201.|[2]Strack RL, et al. Using Spinach-based sensors for fluorescence imaging of intracellular metabolites and proteins in living bacteria. Nat Protoc. 2014 Jan;9 (1) :146-55.|[3]Paige JS, et al. RNA mimics of green fluorescent protein. Science. 2011 Jul 29;333 (6042) :642-6.