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UBE2F Rabbit Polyclonal Antibody (Carrier-free)

Anti-UBE2F Antibody -1. Tested in WB, IHC, ICC/IF, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as , ensuring unmatched performance.

Product Specifications

Product Name Alternative

NCE2; EC:2.3.2.32; NEDD8-conjugating enzyme 2; NEDD8 protein ligase UBE2F; NEDD8 conjugating enzyme 2

UniProt

Q969M7

Reactivity

Human, Mouse, Rat

Cross Reactivity

No cross-reactivity with other proteins

Immunogen

E.coli-derived human UBE2F recombinant protein (Position: M1-R185) . Human UBE2F shares 96.8% and 96.2% amino acid (aa) sequence identity with mouse and rat UBE2F, respectively.

Target

NEDD8-conjugating enzyme UBE2F

Clonality

Polyclonal

Conjugation

Carrier-free

Purification

Immunogen affinity purified.

Concentration

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Dilution

Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human ELISA, 0.1-0.5 μg/ml

Form

Lyophilized

Molecular Weight

21 kDa

Storage Conditions

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Notes

For research use only.

Tested Applications

ELISA, FC, ICC, IHC, WB

Host or Source

Rabbit

Preservative

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Isotype

Rabbit IgG

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