NCL Antibody (CF488)
NCL Antibody (CF488)
Product Specifications
Product Name Alternative
NCL; Nucl; Nucleolin; Protein C23
UniProt
P19338
Reactivity
Human
Immunogen
Recombinant full-length human NCL protein
Target
NCL
Clonality
Monoclonal
Clone
NCL/902
Conjugation
CF488
Field of Research
Cardiovascular Research, Cell Biology
Purification
Protein G
Concentration
Purified Ab conjugated to CF488
Dilution
Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood) ; Immunofluorescence (1:50-1:100) ; (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
76kDa
Storage Conditions
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Notes
For research use only.
Applications Notes
Positive Control: HeLa cells. Breast Cancer
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Isotype
IgG1
Entrez
4691
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