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NCL Antibody (CF488)

NCL Antibody (CF488)

Product Specifications

Product Name Alternative

NCL; Nucl; Nucleolin; Protein C23

UniProt

P19338

Reactivity

Human

Immunogen

Recombinant full-length human NCL protein

Target

NCL

Clonality

Monoclonal

Clone

NCL/902

Conjugation

CF488

Field of Research

Cardiovascular Research, Cell Biology

Purification

Protein G

Concentration

Purified Ab conjugated to CF488

Dilution

Flow Cytometry (5ul per test per one million cells in 0.1ml or 5ul per 100ul of whole blood) ; Immunofluorescence (1:50-1:100) ; (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

76kDa

Storage Conditions

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Notes

For research use only.

Applications Notes

Positive Control: HeLa cells. Breast Cancer

Tested Applications

FC, IF, IHC, WB

Host or Source

Mouse

Preservative

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Isotype

IgG1

Entrez

4691

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