GPC3 Antibody (CF555)

Product Specifications

Product Name Alternative

DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1

UniProt

P51654

Reactivity

Human

Immunogen

A recombinant fragment containing amino acids 511-580 of human glypican-3

Target

GPC3

Clonality

Monoclonal

Clone

SPM595

Conjugation

CF555

Field of Research

Cancer Biology, Cardiovascular Research, Infectious Disease & Virology

Purification

Protein G

Concentration

Purified Ab conjugated to CF555

Dilution

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

~67kDa

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Notes

For research use only.

Applications Notes

Positive Control: 293T cells. Hepatocellular carcinoma

Tested Applications

FC, IF, IHC

Host or Source

Mouse

Preservative

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.