PTPRC Antibody (FITC)
PTPRC Antibody (FITC)
Product Specifications
Product Name Alternative
B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein
UniProt
P08575
Reactivity
Human
Immunogen
Isolated neoplastic cells from T cell lymphoma (2B11) ; and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26) .
Target
PTPRC
Clonality
Monoclonal
Clone
2B11 + PD7/26
Conjugation
FITC
Field of Research
Signal Transduction
Purification
Protein G
Concentration
Purified Ab conjugated to FITC
Dilution
Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.
Form
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
Molecular Weight
180-220kDa
Storage Conditions
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Notes
For research use only.
Applications Notes
Positive Control: Ramos, U-698 or GA-10 cells. Tonsil.
Tested Applications
FC, IF, IHC, WB
Host or Source
Mouse
Preservative
Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. Contact us if you require this Ab in a different format.
Isotype
IgG1
Entrez
5788
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