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PTPRC Antibody (FITC)

PTPRC Antibody (FITC)

Product Specifications

Product Name Alternative

B220, CD45R, GP180, Leukocyte common antigen (LCA), Loc, Ly-5, Lyt-4, Protein tyrosine phosphatase receptor type C (PTPRC), Receptor-type tyrosine-protein phosphatase C, T200 glycoprotein

UniProt

P08575

Reactivity

Human

Immunogen

Isolated neoplastic cells from T cell lymphoma (2B11) ; and human peripheral blood lymphocytes maintained in T cell growth factor (PD7/26) .

Target

PTPRC

Clonality

Monoclonal

Clone

2B11 + PD7/26

Conjugation

FITC

Field of Research

Signal Transduction

Purification

Protein G

Concentration

Purified Ab conjugated to FITC

Dilution

Flow Cytometry (1-2ug/million cells) ; Immunofluorescence (1-2ug/ml) ; Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes) ; Optimal dilution for a specific application should be determined.

Form

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.

Molecular Weight

180-220kDa

Storage Conditions

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Notes

For research use only.

Applications Notes

Positive Control: Ramos, U-698 or GA-10 cells. Tonsil.

Tested Applications

FC, IF, IHC, WB

Host or Source

Mouse

Preservative

Antibody Purified from Bioreactor Concentrate by Protein A/G and conjugated to various reporter molecules. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide. Contact us if you require this Ab in a different format.

Isotype

IgG1

Entrez

5788

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