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Recombinant CA9 Antibody / Carbonic anhydrase 9

Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200) .In normal kidney, gp200 is localized along the brush border of the pars convoluta and pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary and 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Product Specifications

UniProt

Q16790

Reactivity

Human

Immunogen

The extracellular N-terminal proteoglycan-like region of the human protein was used as the immunogen for the recombinant CA9 antibody. The epitope is localized to the amino acids 80-100 region.

Clonality

Recombinant

Clone

RCA9/9937

Conjugation

Unconjugated

Purification

Protein G affinity

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml, Western blot: 2-4ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the recombinant CA9 antibody should be determined by the researcher.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide

Isotype

Mouse IgG2b, kappa

Available Sizes

Frequently Asked Questions

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