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Fibronectin Antibody

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Fibronectins are involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This mAb does not cross-react with MMP-2, MMP-9, or TIMP-2.

Product Specifications

UniProt

P02751

Reactivity

Human

Immunogen

High molecular weight proteins secreted by cultivated human fibroblasts were used as the immunogen for the Fibronectin antibody. The epitope of this mAb is located in the 29kDa N-terminal fibrin- and heparin-binding domain of fibronectin.

Clonality

Monoclonal

Clone

616

Conjugation

Unconjugated

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Fibronectin antibody should be determined by the researcher.1.The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P

Host or Source

Mouse

Preservative

Prediluted in 1X PBS, 0.1 mg/ml rAlbumin, 0.05% sodium azide; For IHC use only

Isotype

Mouse IgG2a, kappa

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