Z-VAD-FMK

Z-VAD-FMK is a pan-caspase inhibitor and also an ICE-like protease inhibitor, which inhibits apoptosis by preventing the processing of CPP32 to its active form. Z-VAD-FMK sensitivity varies primarily due to differential expression of receptor-interacting protein 1 (RIP1) . Z-VAD-FMK limits the cryopreservation-induced apoptosis by reducing caspase-3 activity of in vitro produced bovine embryos. Z-VAD-FMK is immunosuppressive in vitro and inhibits T cell proliferation without blocking the processing of caspase-8 and caspase-3. Z-VAD-FMK leads to a decrease in intracellular glutathione (GSH) with a concomitant increase in reactive oxygen species (ROS) levels in activated T cells. Z-VAD-FMK is due to oxidative stress via the depletion of GSH. Z-VAD-FMK can be used for the study of acute pancreatitis[1][2][3][4][5][6][7].

Product Specifications

CAS Number

[161401-82-7]

Product Name Alternative

Z-VAD (OH) -FMK

UNSPSC

12352005

Hazard Statement

H302, H315, H319, H335

Target

Apoptosis; Caspase; Glutathione Peroxidase; Reactive Oxygen Species (ROS) ; RIP kinase

Type

Reference compound

Related Pathways

Apoptosis; Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB

Field of Research

Cancer; Inflammation/Immunology

Assay Protocol

https://www.medchemexpress.com/z-vad-fmk-1.html

Purity

99.78

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C(N[C@H](C(N[C@@H](C)C(N[C@@H](CC(O)=O)C(CF)=O)=O)=O)C(C)C)OCC1=CC=CC=C1

Molecular Formula

C21H28FN3O7

Molecular Weight

453.46

Precautions

H302, H315, H319, H335

References & Citations

[1]Liu HR, et al. Antiproliferative activity of the total saponin of Solanum lyratum Thunb in Hela cells by inducing apoptosis. Pharmazie. 2008 Nov;63 (11) :836-42.|[2]Slee EA, et al. Benzyloxycarbonyl-Val-Ala-Asp (OMe) fluoromethylketone (Z-VAD.FMK) inhibits apoptosis by blocking the processing of CPP32. Biochem J. 1996 Apr 1;315 (Pt 1) (Pt 1) :21-4.|[3]Martinet W, et al. Z-VAD-FMK-induced non-apoptotic cell death of macrophages: possibilities and limitations for atherosclerotic plaque stabilization. Autophagy. 2006 Oct-Dec;2 (4) :312-4.|[4]Pero ME, Zullo G, Esposito L, Iannuzzi A, Lombardi P, De Canditiis C, Neglia G, Gasparrini B. Inhibition of apoptosis by caspase inhibitor Z-VAD-FMK improves cryotolerance of in vitro derived bovine embryos. Theriogenology. 2018 Mar 1;108:127-135. |[5]Lawrence CP, Chow SC. Suppression of human T cell proliferation by the caspase inhibitors, Z-VAD-FMK and z-IETD-FMK is independent of their caspase inhibition properties. Toxicol Appl Pharmacol. 2012 Nov 15;265 (1) :103-12.|[6]Rajah T, Chow SC. The inhibition of human T cell proliferation by the caspase inhibitor Z-VAD-FMK is mediated through oxidative stress. Toxicol Appl Pharmacol. 2014 Jul 15;278 (2) :100-6.|[7]He M, et al. Effects of Z-VaD-Ala-Asp-Fluoromethyl Ketone (Z-VAD-FMK) and Acetyl-Asp-Glu-Val-Asp-Aldehyde (Ac-DEVD-CHO) on Inflammation and Mucus Secretion in Mice Exposed to Cigarette Smoke. Int J Chron Obstruct Pulmon Dis. 2023 Feb 5;18:69-78.|[8]Liu M, et al. Caspase inhibitor zVAD-fmk protects against acute pancreatitis-associated lung injury via inhibiting inflammation and apoptosis. Pancreatology. 2016 Sep-Oct;16 (5) :733-8.|[9]Davies CW, et al. The co-crystal structure of ubiquitin carboxy-terminal hydrolase L1 (UCHL1) with a tripeptide fluoromethyl ketone (Z-VAE (OMe) -FMK) . Bioorg Med Chem Lett. 2012 Jun 15;22 (12) :3900-4.