Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68 Protein, His Tag
Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (RE8-A5243) is expressed from human 293 cells (HEK293) . It contains AA Met 1 - Leu 536 (Accession # P03132-1) .
Product Specifications
Background
Adeno-associated virus (AAV) is a single-stranded DNA virus, and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid and a single-stranded DNA genome of 4.7 kb in length, with two 'T' terminal inverted repeats (ITRs) at each end of the AAV genome. The rep genes in the AAV genome encode four proteins associated with viral replication, Rep78, Rep68, Rep52, and Rep40.
Specifications
This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 62.8 kDa. The protein migrates as 75-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P03132-1
Expression Region
Met 1 - Leu 536
Host
HEK293
Target
Rep 68
Conjugation
Unconjugated
Tag
N-10xHis
Source
Adeno-associated virus 2
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
95%
Bioactivity
Immobilized Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (Cat. No. RE8-A5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Mouse anti‑Adeno‑Associated Virus AAV2 Rep68 Antibody with a linear range of 0.1-4 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
62.8 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
100ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/RE8-A5243.pdf
Species
Adeno-associated virus 2
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