Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68 Protein, His Tag

Adeno-associated virus (AAV) is a single-stranded DNA virus, and the current scientific consensus is that it does not cause any human disease. It consists of a protein capsid and a single-stranded DNA genome of 4.7 kb in length, with two 'T' terminal inverted repeats (ITRs) at each end of the AAV genome. The rep genes in the AAV genome encode four proteins associated with viral replication, Rep78, Rep68, Rep52, and Rep40.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 62.8 kDa. The protein migrates as 75-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P03132-1

Expression Region

Met 1 - Leu 536

Host

HEK293

Target

Rep 68

Conjugation

Unconjugated

Tag

N-10xHis

Source

Adeno-associated virus 2

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Adeno-associated virus 2 (isolate Srivastava/1982) (AAV-2) Rep 68, His Tag (Cat. No. RE8-A5243) at 1 μg/mL (100 μL/well) can bind Monoclonal Mouse anti‑Adeno‑Associated Virus AAV2 Rep68 Antibody with a linear range of 0.1-4 ng/mL (QC tested).