PolQi2

PolQi2 is a PolΘ inhibitor that targets and inhibits alt-EJ (alternative end-joining) repair by inhibiting the helicase domain at the N-terminus of PolΘ. PolQi2 enhances the precision and integration efficiency of gene editing at different loci and in various cell lines. Furthermore, the combined use of PolQi2 with DNA-PK inhibitors reduces the off-target effects of Cas9. PolQi2 can be used in gene editing research[1][2].

Product Specifications

CAS Number

[2565638-16-4]

UNSPSC

12352005

Target

DNA/RNA Synthesis

Type

Reference compound

Related Pathways

Cell Cycle/DNA Damage

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/polqi2.html

Purity

99.65

Solubility

DMSO : 70 mg/mL (ultrasonic)

Smiles

O=C(C1=CC=NC=C1C2=C(OC)C=CC=C2)NC3=NN=C(S3)OCC4=NC=C(C=C4)Cl

Molecular Formula

C21H16ClN5O3S

Molecular Weight

453.90

References & Citations

[1]imberger S, et al. Simultaneous inhibition of DNA-PK and Polθ improves integration efficiency and precision of genome editing. Nat Commun. 2023 Aug 14;14 (1) :4761.|[2]Antoniou P, et al. Improved nuclease-based prime editing by DNA repair modulation and pegRNA engineering[J]. BioRxiv, 2024: 2024.02. 01.578377.