N-Ethylmaleimide

N-Ethylmaleimide (NEM) derives from maleic acid, it can alkylates free sulfhydryl. N-Ethylmaleimide is an irreversible cysteine protease inhibitor. N-ethylmaleimide specific inhibits phosphate transport in mitochondria. N-Ethylmaleimide inhibits prolyl endopeptidase with an IC50 value of 6.3 μM. N-Ethylmaleimide can be used to modify cysteine residues in proteins and peptides[1][2][3].

Product Specifications

CAS Number

[128-53-0]

Product Name Alternative

NEM

UNSPSC

12352005

Hazard Statement

H300, H311, H314, H317

Target

Apoptosis; Cathepsin; Deubiquitinase

Type

Reference compound

Related Pathways

Apoptosis; Cell Cycle/DNA Damage; Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/n-ethylmaleimide.html

Purity

99.97

Solubility

DMSO : 100 mg/mL (ultrasonic) |Ethanol : 12.5 mg/mL (ultrasonic) |H2O : 50 mg/mL (ultrasonic)

Smiles

O=C(C=C1)N(CC)C1=O

Molecular Formula

C6H7NO2

Molecular Weight

125.13

Precautions

H300, H311, H314, H317

References & Citations

[1]Moriyama A, et al. Porcine muscle prolyl endopeptidase and its endogenous substrates. J Biochem. 1988 Jul;104 (1) :112-7.|[2]Yellaturu CR, et al. N-Ethylmaleimide inhibits platelet-derived growth factor BB-stimulated Akt phosphorylation via activation of protein phosphatase 2A. J Biol Chem. 2002 Oct 18;277 (42) :40148-55.|[3]Matsuda H, et al. Roles of capsaicin-sensitive sensory nerves, endogenous nitric oxide, sulfhydryls, and prostaglandins in gastroprotection by momordin Ic, an oleanolic acid oligoglycoside, on ethanol-induced gastric mucosal lesions in rats. Life Sci. 1999;65 (2) :PL27-32.