DAF-FM DA
DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO) . DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm) [1].
Product Specifications
CAS Number
254109-22-3
Product Name Alternative
Diaminofluorescein-FM diacetate
UNSPSC
12171500
Hazard Statement
H315, H319, H335
Target
Fluorescent Dye
Type
Dye Reagents
Related Pathways
Others
Field of Research
Cancer
Assay Protocol
https://www.medchemexpress.com/DAF-FM-DA.html
Purity
98.32
Solubility
DMSO : 2.5 mg/mL (ultrasonic; warming)
Smiles
O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5
Molecular Formula
C25H18F2N2O7
Molecular Weight
496.42
Precautions
H315, H319, H335
References & Citations
[1]Zhi Xu, et al. RelB-activated GPX4 inhibits ferroptosis and confers tamoxifen resistance in breast cancer. Redox Biol. 2023 Dec:68:102952.|[2]Ann Huysseune, et al. Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection. Biomolecules. 2023 Dec 12;13 (12) :1780.
Shipping Conditions
Blue Ice
Storage Conditions
-20°C (Powder, protect from light)
Scientific Category
Dye Reagents
Clinical Information
No Development Reported
Available Sizes
Frequently Asked Questions
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