DAF-FM DA

DAF-FM DA (Diaminofluorescein-FM diacetate) is a fluorescent probe for the detection and bioimaging of nitric oxide (NO) . DAF-FM DA spontaneously crosses the plasma membrane and is subsequently cleaved by esterases to generate intracellular DAF-FM (Ex/Em=495/515 nm) [1].

Product Specifications

CAS Number

[254109-22-3]

Product Name Alternative

Diaminofluorescein-FM diacetate

UNSPSC

12171500

Hazard Statement

H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/DAF-FM-DA.html

Purity

98.32

Solubility

DMSO : 2.5 mg/mL (ultrasonic; warming)

Smiles

O=C1OC2(C3=C(OC4=C2C=C(F)C(OC(C)=O)=C4)C=C(OC(C)=O)C(F)=C3)C5=C1C(N)=C(NC)C=C5

Molecular Formula

C25H18F2N2O7

Molecular Weight

496.42

Precautions

H315, H319, H335

References & Citations

[1]Zhi Xu, et al. RelB-activated GPX4 inhibits ferroptosis and confers tamoxifen resistance in breast cancer. Redox Biol. 2023 Dec:68:102952.|[2]Ann Huysseune, et al. Bone Formation in Zebrafish: The Significance of DAF-FM DA Staining for Nitric Oxide Detection. Biomolecules. 2023 Dec 12;13 (12) :1780.