CD1a Mouse Monoclonal Antibody
The CD1a Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD1a .Purification: The antibody was purified by affinity chromatography.
Product Specifications
Product Name Alternative
T-cell surface glycoprotein CD1a; T-cell surface antigen T6/Leu-6; hTa1 thymocyte antigen; CD1a
UniProt
P06126
Reactivity
Human
Immunogen
Native purified human CD1a
Target
CD1A
Clonality
Monoclonal
Clone
BJK361
Conjugation
Unconjugated
Purification
The antibody was purified by affinity chromatography.
Form
Liquid
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured) .
Tested Applications
FC, IF
Host or Source
Mouse
Preservative
PBS, pH 7.3, and 0.02% sodium azide.
Isotype
Mouse IgG1 kappa
Entrez
909
Available Sizes
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